Video Recording

Role of Chloramphenicol Acetyltransferase (CAT) Enzyme for Early Detection of Chloramphenicol Resistant Salmonella typhi

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Microbial resistance to antibiotic is a major problem in the treatment of infectious diseases. The purpose of this study was to identification the role of CAT enzyme for early detection of chloramphenicol resistant Salmonella typhi. Observation was performed on Salmonella typhi isolated from Dr. Sutomo Hospital, Surabaya Health Laboratory, and Microbiology Laboratory-Airlangga University, Surabaya in 2009 and had been sub- cultured. The sub-culture was then tested for its susceptibility to chloramphenicol by using anti-chloramphenicol acetyl transferase (CAT) antibody from Sigma (catalog number C.9336). Susceptibility test using dilution and diffusion methods proved that resistant Salmonella typhi could change to sensitive and vice versa. It seems that the CAT enzyme can bind anti-CAT so reversing the resistant Salmonella typhi into sensitive and conversely, sensitive Salmonella typhi into resistant one

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Indonesia

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NONE

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Subject(s)

CAT enzyme, chloramphenicol, resistant Salmonella

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